Blood components

Concentration of leucodepleted red blood cells with added solution (concentration of filtered red blood cells with added solution)

DEFINITION

Red blood cells from a total blood unit, without plasma and filtered to eliminate most of the leucocytes, in an added solution (SAG-Manitol).

HOW IT IS OBTAINED

The total blood is centrifuged to separate the plasma (and also sometimes the leucoplatelet layer), from which the concentrations of platelets are made. The red blood cell concentrations are obtained and are mixed with the added solution. Filtering can be carried out from total blood or red blood cell concentrations.

COMPOSITION AND CHARACTERISTICS

  • Volume: the average volume of red blood cell concentrates is 289±25 ml; in all cases it is above 242 ml.
  • Haemoglobin: the average amount of Haemoglobin is 56.3±6.9 g; in all cases it is above 43 g.
  • Haematocrit: the average value of the haematocrit is 56.8±2.9%; it is always above 45%.
  • Leucocytes: the average final content of leucocytes is 0.24± 0.38 x 106; it is always below 1 x106.

ANALYSIS RESULTS

  • ABO group and Rh factor.
  • Extensive eritrocitari phenotype in some cases.
  • Irregular antibodies: negative for clinically significant antibodies in the usage conditions of this product.
  • Surface antigen of the hepatitis B virus (HBs Ag): negative.
  • Antibodies against the hepatitis C virus (Anti-HCV): negative.
  • Antibodies against the Immunodeficiency virus (Anti-HIV 1+2): negative.
  • Syphilis serology: negative.
  • NAT of HCV (PCR): negative.
  • NAT of HIV (PCR): negative.
  • NAT of VHB (PCR): negative.
  • Anti-Trypanosoma cruzi antibodies: negative (only carried out on donors with a risk of being carriers).

RECIPIENT

Collapsible plastic bags.

IDENTIFICATION

With labels containing the following data: name of the centre, type of product, ABO group and Rh (D) factor, extensive phenotype if applicable, unit number, date of extraction, expiry date, results of analysis, type of anticoagulant, preservation and administration conditions.

STORAGE

Between 2ºC and 6ºC.

EXPIRY DATE

42 days.

HANDLING

The concentrations of red blood cells can be irradiated and divided into subunits.