Clinical information
Clinical utility
Some hematopoietic cell apheresis requires plasma reduction. This process may be performed when:
1. A product must be frozen: to reduce the volume and thus the amount of space and *dimethyl sulfoxide* (DMSO).
2. There is minor ABO incompatibility with isohemagglutinins above a safety threshold.
3. There is an excess volume of a product (> 20 ml/kg patient).
Method
The volume reduction service carried out by the Cell Therapy Service (Cell Therapy Laboratory) is automated and performed in a closed system to reduce the time and risk of contamination.
The process consists of centrifugation and separation of the cellular components, guaranteeing the preservation of the cell layer. The amount of plasma removal will vary depending on clinical needs.
Reference values
The loss of cellularity of the volume reduction process must be less than 10%.
Specimen information
Product: HPC/MNC by apheresis.
Tube: not applicable.
Volume: less than 200 ml.
Shelf life: 72 hours.
Transport instructions: 2-8 °C.
Storage instructions: 2-8 °C.
Administrative information
BST code: BT104.
Description of the test: applies when the progenitor cell donor has a high titer of specific isohemagglutinins against the recipient's blood group (minor incompatibility). In this case, the processing laboratory performs depletion of the residual plasma in a validated procedure (fee BT104). It is also applicable if it is necessary to perform volume reduction of the HPC product prior to infusion.
Synonyms: HPC/MNC-plasma depleted.
Section: Cell Therapy Service. Cell Therapy Laboratory.
Service: this fee also applies if it is necessary to perform volume reduction of the HPC product prior to infusion.
References
Areman, EM, Loper, K, editors. Cellular therapy: Principles, methods, and regulations. 2a ed. Bethesda (MD): AABB Press, 2016.
Quality
The BST has ISO 9001, ISO 14001, OSHAS 18001 and European Seal of Excellence 500+ quality certifications. The BST is accredited by CAT, JACIE-FACT, FACT-NETCORD and EFI. It complies with the guidelines of good manufacturing and good distribution practices.
