Test Catalog

Platelet Antigen HPA-4a

Code: 0606

Type: Estudis plaquetaris

Clinical information

Diagnostic Utility:

  • This determination is useful in the typing of patients or donors where it is of interest to confirm the presence or absence of this antigen.
  • There are different techniques for typing HPA antigens, with molecular biology techniques currently being the most commonly used.

Method:

Determination of the HPA-4a genotype

Prior to determining the genotype, DNA extraction from a blood sample in EDTA is required.

The study of the HPA-4a platelet genotype is carried out using the SSP-PCR technique, with specific primers for the HPA-4a allele in a uniplex.

Results:

The results are: 

  • Positive
  • Negative

Response Time:

10 working days

Specimen information

Sample: Peripheral blood in EDTA
Sample volume: 1 tube of 5 ml of EDTA K3
Stability: At room temperature or at 4°C: 7 days
Transport instructions: Preferably at room temperature
Reasons for rejection: Highly hemolyzed sample

Administrative information

BST Code: 0606
Test Description: Platelet Antigen HPA-4a
Synonyms: --
Section:
Immunohematology
BST Rate: Check the updated rates here.

Profiles: Not included in any profile

References

G.Cavanagh, A. N. Dunn, C. E. Chapman i P. Metcalfe. " HPA genotyping by PCR sequence-specific priming (PCR-SSP): a streamlined method for rapid routine investigations". Transfusion Medicine, 7: 41-45 (1997).