Test Catalog

Platelet Antigen HPA-1a

Code: 0600

Type: Estudis plaquetaris

Clinical information

Diagnostic Utility:

  • Typing of platelet antigen HPA-1a is useful for identifying blood donors negative for this antigen, to select them as potential platelet donors.
  • It is also useful to identify semen donors negative for HPA-1a, who may be valid for assisted reproduction in women alloimmunized against the HPA-1a antigen.
  • There are different techniques for typing HPA antigens, with molecular biology techniques currently being the most used.

Method:

Determination of the HPA-1a genotype

Prior to determining the genotype, DNA extraction from a blood sample in EDTA is required.

The study of the HPA-1a platelet genotype is carried out using the SSP-PCR technique, with specific primers for the HPA-1a allele in a uniplex reaction.

Results:

The results are: 

  • Positive
  • Negative

Response Time:

10 working days

Specimen information

Sample: Peripheral blood in EDTA
Sample volume: 1 tube of 5 ml of EDTA K3
Stability: At room temperature or at 4°C: 7 days
Transport instructions: Preferably at room temperature
Reason for rejection: Highly hemolyzed sample

Administrative information

BST Code: 0600
Test Description: Platelet Antigen HPA-1a
Synonyms: --
Section:
Immunohematology
BST Fee: Check the updated fees here.

Profiles:

The 0600 Platelet Antigen HPA-1a test can be requested individually. It is also performed within the following profiles:

Profile Included Tests
Semen Donor Profile

0600 Platelet Antigen HPA-1a

0601 Platelet Antigen HPA-1b

References

G.Cavanagh, A. N. Dunn, C. E. Chapman i P. Metcalfe. " HPA genotyping by PCR sequence-specific priming (PCR-SSP): a streamlined method for rapid routine investigations". Transfusion Medicine, 7: 41-45 (1997).