Clinical information
Diagnostic Utility:
- Typing of platelet antigen HPA-1a is useful for identifying blood donors negative for this antigen, to select them as potential platelet donors.
- It is also useful to identify semen donors negative for HPA-1a, who may be valid for assisted reproduction in women alloimmunized against the HPA-1a antigen.
- There are different techniques for typing HPA antigens, with molecular biology techniques currently being the most used.
Method:
Determination of the HPA-1a genotype
Prior to determining the genotype, DNA extraction from a blood sample in EDTA is required.
The study of the HPA-1a platelet genotype is carried out using the SSP-PCR technique, with specific primers for the HPA-1a allele in a uniplex reaction.
Results:
The results are:
- Positive
- Negative
Response Time:
10 working days
Specimen information
Sample: Peripheral blood in EDTA
Sample volume: 1 tube of 5 ml of EDTA K3
Stability: At room temperature or at 4°C: 7 days
Transport instructions: Preferably at room temperature
Reason for rejection: Highly hemolyzed sample
Administrative information
BST Code: 0600
Test Description: Platelet Antigen HPA-1a
Synonyms: --
Section: Immunohematology
BST Fee: Check the updated fees here.
Profiles:
The 0600 Platelet Antigen HPA-1a test can be requested individually. It is also performed within the following profiles:
Profile | Included Tests |
---|---|
Semen Donor Profile |
0600 Platelet Antigen HPA-1a 0601 Platelet Antigen HPA-1b |
References
G.Cavanagh, A. N. Dunn, C. E. Chapman i P. Metcalfe. " HPA genotyping by PCR sequence-specific priming (PCR-SSP): a streamlined method for rapid routine investigations". Transfusion Medicine, 7: 41-45 (1997).