Test Catalog

Platelet Antigen HPA-3b

Code: 0605

Type: Estudis plaquetaris

Clinical information

Diagnostic Utility:

  • This determination is useful in the typing of patients or donors where it is of interest to confirm the presence or absence of this antigen.
  • There are different techniques for typing HPA antigens, with molecular biology techniques currently being the most widely used.

Method:

Determination of the HPA-3b genotype
Prior to determining the genotype, DNA extraction must be performed from a blood sample in EDTA.
The study of the platelet genotype HPA-3b is carried out using the SSP-PCR technique, with specific primers for the HPA-3b allele in a uniplex reaction.

Results:

The results are:

Positive
Negative

Turnaround Time:

10 working days

Specimen information

Sample: Peripheral blood in EDTA
Sample volume: 1 tube of 5 ml of EDTA K3
Stability: At room temperature or at 4°C: 7 days
Transport instructions: Preferably at room temperature
Reason for rejection: Highly hemolyzed sample

Administrative information

BST Code: 0605
Test Description: Platelet Antigen HPA-3b
Synonyms: --
Section:
Immunohematology
BST Rate: Check the updated rates here.

Profiles: Not included in any profile

References

G.Cavanagh, A. N. Dunn, C. E. Chapman i P. Metcalfe. " HPA genotyping by PCR sequence-specific priming (PCR-SSP): a streamlined method for rapid routine investigations". Transfusion Medicine, 7: 41-45 (1997).