Clinical information
Diagnostic utility:
High-resolution HLA typing allows us to determine the HLA typing at an allelic level (4 digits). The typing of the HLA-DPA1 locus is of special interest in donors in order to assess the presence of donor-specific antibodies in transplant patients.
Method:
The PCR-NGS technique (Long strategy) enables high-resolution HLA typing of class I and II HLA genes and non-classical HLA genes. This technique consists of several phases: amplification of the HLA genes of interest by PCR, library preparation, sequencing, and result analysis. With the Long strategy (long fragments), the complete gene sequence is obtained.
Reference values
With high-resolution typing, two results are obtained for each HLA gene, for example: HLA-DPA1*01:03 DPA1*02:01.
Diagnostic algorithm:
Not applicable.
Turnaround time:
The turnaround time for high-resolution HLA typing depends on the sample origin:
- Patient or family members of a patient candidate for hematopoietic stem cell transplantation (20 working days).
- Volunteer donor registry (20 working days).
- Expansion of HLA typing in volunteer donors (10 working days).
- Umbilical cord blood units registry (20 working days).
- Verification of umbilical cord blood units (20 working days).
- Pre-transplant donor and recipient confirmation (20 working days).
Specimen information
Sample: Whole Blood
Tube: EDTA K3 4 ml Tube
Minimum Volume: 500µl.
Stability:
- In refrigeration: 2 weeks.
- At -20°C: from 2 weeks onwards.
Transport Instructions: Preferably at room temperature
Rejection Reason: Clotted sample
Administrative information
BST Code: LRD2061
Test Description: High-resolution HLA-DPA1 typing
Synonyms: High HLA-DPA1
Section: Histocompatibility and Immunogenetics
BST Fee: Check the updated fees here.
Profiles: Not applicable.
References
Manual dels "Standards for Histocompatibility Testing" de l'EFI (darrera versió).