Concentrations of red blood cells preserved frozen at -80ºC.
Firstly, glycerol is added to the red blood cell concentration to obtain a 40% intraerythrocyte concentration of glycerol, and then it is placed in an electric freezer at -80ºC.
Before use, the red blood cell concentration is thawed in a Bain Marie at 37ºC and then undergoes a process of deglycerolisation by adding a hypertonic solution of 12% NaCl. It is then washed with a solution of 0.9% NaCl and 0.2% glucose to obtain a suspension of red blood cells in this solution.
Red blood cells: During the freezing and thawing of red blood cell concentrations, the loss of cells is no more than 20%.
Haemoglobin free from serum: less than 0.2 g/dl.
Collapsible plastic bags. During the preservation at -80ºC, these bags are placed in a cardboard box..
With labels containing the following data: name of the centre, type of product, ABO group and Rh (D) factor, unit number, date of extraction, expiry date, results of analysis, type of anticoagulant, preservation and administration conditions.
Frozen red blood cell concentrations at -80ºC can be kept for 10 years.
After thawing, they must be used within 24 hours.