Blood components

Fresh deactivated plasma

DEFINITION

Fresh plasma that has been treated with methylene blue to significantly reduce its content of virus with lipidic capsule.

HOW IT IS OBTAINED

The plasma units are filtered through a porous filter with a diameter of 0.8 to 1.2 microns to eliminate the leucocytes. Then, 83 micrograms of methylene blue is added per 235-310 ml of plasma and it is exposed to ultraviolet light at an intensity of 30 J/cm2 for 30 minutes.

COMPOSITION AND CHARACTERISTICS

Volume:

• variable according to the origin.

Average contents:

• Factor VIII 0.79 UI/ml
• Factor V 0.88 UI/ml
• Factor II 0.94 UI/ml
• Fibrinogen 2.20 g/l

ANALYSIS RESULTS

• ABO group and Rh factor
• Irregular antibodies: negative.
• Surface antigen of the hepatitis B virus (HBs Ag): negative.
• Antibodies against the hepatitis C virus (Anti-HCV): negative.
• Antibodies against the Immunodeficiency virus (Anti-HIV 1+2): negative.
• Syphilis serology: negative.
• NAT of HCV (PCR): negative.
• NAT of HIV (PCR): negative.
• NAT of VHB (PCR): negative.
• Anti-Trypanosoma cruzi antibodies: negative (only carried out on donors with a risk of being carriers).

RECIPIENT

Collapsible plastic bags.

IDENTIFICATION

With labels containing the following data: name of the centre, type of product, ABO group and Rh (D) factor, unit number, date of extraction, expiry date, results of analysis, type of anticoagulant, preservation and administration conditions.

CONSERVATION

At -30ºC.

EXPIRY DATE

3 years from the moment the blood is extracted.

HANDLING

The fresh plasma units treated with methylene blue can be divided into subunits.