Clinical information
Clinical utility
Bone marrow collection for hematopoietic progenitor cell transplantation (HPCT) contains a high number of red blood cells (hematocrit around 30-35%) and plasma.
In some situations, such as donor/recipient ABO incompatibility, excess volume greater than 20 ml/kg/patient, cryopreservation or history of allergic reactions, it is necessary to reduce the number of red cells and plasma and recover the fraction of nucleated cells (buffy coat) from bone marrow where the hematopoietic progenitor cells (HPCs) are found.
Method
Automated bone marrow (BM) buffy coat processing is faster and less operator dependent compared to manual processing. Moreover, since it is carried out in a closed system, the risk of contamination is lower.
The Banc de Sang i Teixits (Blood and Tissue Bank, BST) uses the Sepax system, which consists of a centrifuge system and disposable equipment. The centrifuge allows the separation of BM blood into different components using an optical light signal system. Following several repeat cycles depending on the initial volume, the device delivers the components in separate bags (buffy coat, red blood cells, plasma).
Reference values
The expected recovery of nucleated cells is > 75%.
Specimen information
Product: BM NC.
Tube: perform quality controls.
Volume: desired volume up to a maximum of 1500 ml.
Shelf life: 72 hours from the time it was obtained.
Transport instructions: 2-8 °C.
Storage instructions: 2-8 °C.
Administrative information
BST code: BT103.
Test description: automated processing of BM hematopoietic progenitor cells (HPCs).
Synonyms: NC-BM.
Section: Cell Therapy Service. Cell Therapy Laboratory.
Service: the fee includes obtaining the buffy coat (nucleated cells) from BM donation.
References
Areman, E. M.; Loper, K., editors. Cellular therapy: Principles, methods, and regulations. 2nd ed. Bethesda (MD): AABB Press, 2016.
Quality
The BST has ISO 9001, ISO 14001, OSHAS 18001 and European Seal of Excellence 500+ quality certifications. The BST is accredited by CAT, JACIE-FACT, FACT-NETCORD and EFI. It complies with the guidelines of good manufacturing and good distribution practices.
